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hy u00451  (MedChemExpress)


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    MedChemExpress hy u00451
    Hy U00451, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy+u00451/ATP-Red+1/pmc12924746-22-6-3
    Average 95 stars, based on 22 article reviews
    hy u00451 - by Bioz Stars, 2026-09
    95/100 stars

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    PPARγ activation maintains metabolic homeostasis under oxidative stress (A) Measurement of mitochondrial <t>ATP</t> <t>levels</t> in 2-cell and blastocyst embryos. (B) Quantification of ATP content from (A). (C) Representative images of lipid peroxidation (C11 BODIPY 581/591 staining) in 2-cell embryos. Green fluorescence indicates oxidized lipid, red indicates non-oxidized lipid. (D) Representative images of lipid peroxidation in blastocysts. (E) Quantification of the green/red fluorescence ratio (oxidized/non-oxidized) in 2-cell embryos from (C). (F) Quantification of the green/red fluorescence ratio in blastocysts from (D). Data are represented as mean ± SEM; n > 5 per group). All data from 3 independent biological replicates. ns p ≥ 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by one-way ANOVA with post-hoc test. Scale bar = 20 μm.
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    PPARγ activation maintains metabolic homeostasis under oxidative stress (A) Measurement of mitochondrial ATP levels in 2-cell and blastocyst embryos. (B) Quantification of ATP content from (A). (C) Representative images of lipid peroxidation (C11 BODIPY 581/591 staining) in 2-cell embryos. Green fluorescence indicates oxidized lipid, red indicates non-oxidized lipid. (D) Representative images of lipid peroxidation in blastocysts. (E) Quantification of the green/red fluorescence ratio (oxidized/non-oxidized) in 2-cell embryos from (C). (F) Quantification of the green/red fluorescence ratio in blastocysts from (D). Data are represented as mean ± SEM; n > 5 per group). All data from 3 independent biological replicates. ns p ≥ 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by one-way ANOVA with post-hoc test. Scale bar = 20 μm.

    Journal: iScience

    Article Title: PPARγ activation rescues oxidative stress-induced embryonic arrest by suppressing Wnt/β-catenin signaling via GSK3β upregulation

    doi: 10.1016/j.isci.2026.114870

    Figure Lengend Snippet: PPARγ activation maintains metabolic homeostasis under oxidative stress (A) Measurement of mitochondrial ATP levels in 2-cell and blastocyst embryos. (B) Quantification of ATP content from (A). (C) Representative images of lipid peroxidation (C11 BODIPY 581/591 staining) in 2-cell embryos. Green fluorescence indicates oxidized lipid, red indicates non-oxidized lipid. (D) Representative images of lipid peroxidation in blastocysts. (E) Quantification of the green/red fluorescence ratio (oxidized/non-oxidized) in 2-cell embryos from (C). (F) Quantification of the green/red fluorescence ratio in blastocysts from (D). Data are represented as mean ± SEM; n > 5 per group). All data from 3 independent biological replicates. ns p ≥ 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by one-way ANOVA with post-hoc test. Scale bar = 20 μm.

    Article Snippet: ATP: ATP levels were measured using ATP Red-1 (MCE, HY-U00451).

    Techniques: Activation Assay, Staining, Fluorescence